hPLMA Photopolymerizable hydrogel is a human based, xeno-free, bioactive platelet lysates-derived platform that provides physiologically relevant microenvironments for cell culture. The precursor solution is easily prepared by reconstituting hPLMA lyophilizate in an aqueous solution with a photoinitiator. Afterwards it can be cured upon light exposure to form a hydrogel with tuneable mechanical properties. hPLMA Photopolymerizable hydrogels provide great control over gelation time and matrix stiffness, enabling researchers to create the cell culture environment that better fits their needs.